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bont a hc hrp  (Cytiva Europe)


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    Cytiva Europe bont a hc hrp
    Electron microscopy of axon channels reveals that BoNT/A-Hc is retrogradely transported by autophagosomes. A, Representative axon bundles from unlabeled neurons with autophagosomes (a1 and a2, boxes) and mitochondria (arrowheads) along the microtubule tracks. Scale bar, 500 nm. B, C, Quantification of autophagosomes (B) and mitochondria (C) observed along the axon bundles in the indicated conditions; n = 3 independent microfluidic devices. *p < 0.05; n.s. not significant, Student’s t test. D, Representative electron microscopy of axon bundles of neurons cultured in microfluidic devices with (right) or <t>without</t> <t>(left)</t> <t>BoNT/A-Hc-HRP</t> added to the nerve terminal chambers under high K+ conditions. Autophagosomes are indicated by arrowheads. Scale bar, 500 nm. E, Quantification of BoNT/A-Hc-HRP-containing autophagosome as shown in D; n = 3. **p < 0.01, Student’s t test. F, Representative LC3 and βIII-tubulin immunostaining of hippocampal neurons cultured in microfluidic devices and fixed at indicated times after stimulation. Boxed regions are shown in G; arrowheads indicate nerve terminals. H, Quantification of the level of LC3 fluorescence normalized to βIII-tubulin; n = 6 from 2 independent cultures. ***p < 0.001; **p < 0.01, Student’s t test. I, Representative LC3 and β-actin (loading control) immunoblots. J, Quantification of I; n = 3. *p < 0.05, Student’s t test.
    Bont A Hc Hrp, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 733 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bont+a+hc+hrp/PD+MiniTrap+G-25/pmc04787026-49-2-11
    Average 96 stars, based on 733 article reviews
    bont a hc hrp - by Bioz Stars, 2026-09
    96/100 stars

    Images

    1) Product Images from "Control of Autophagosome Axonal Retrograde Flux by Presynaptic Activity Unveiled Using Botulinum Neurotoxin Type A"

    Article Title: Control of Autophagosome Axonal Retrograde Flux by Presynaptic Activity Unveiled Using Botulinum Neurotoxin Type A

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    doi: 10.1523/JNEUROSCI.3757-14.2015

    Electron microscopy of axon channels reveals that BoNT/A-Hc is retrogradely transported by autophagosomes. A, Representative axon bundles from unlabeled neurons with autophagosomes (a1 and a2, boxes) and mitochondria (arrowheads) along the microtubule tracks. Scale bar, 500 nm. B, C, Quantification of autophagosomes (B) and mitochondria (C) observed along the axon bundles in the indicated conditions; n = 3 independent microfluidic devices. *p < 0.05; n.s. not significant, Student’s t test. D, Representative electron microscopy of axon bundles of neurons cultured in microfluidic devices with (right) or without (left) BoNT/A-Hc-HRP added to the nerve terminal chambers under high K+ conditions. Autophagosomes are indicated by arrowheads. Scale bar, 500 nm. E, Quantification of BoNT/A-Hc-HRP-containing autophagosome as shown in D; n = 3. **p < 0.01, Student’s t test. F, Representative LC3 and βIII-tubulin immunostaining of hippocampal neurons cultured in microfluidic devices and fixed at indicated times after stimulation. Boxed regions are shown in G; arrowheads indicate nerve terminals. H, Quantification of the level of LC3 fluorescence normalized to βIII-tubulin; n = 6 from 2 independent cultures. ***p < 0.001; **p < 0.01, Student’s t test. I, Representative LC3 and β-actin (loading control) immunoblots. J, Quantification of I; n = 3. *p < 0.05, Student’s t test.
    Figure Legend Snippet: Electron microscopy of axon channels reveals that BoNT/A-Hc is retrogradely transported by autophagosomes. A, Representative axon bundles from unlabeled neurons with autophagosomes (a1 and a2, boxes) and mitochondria (arrowheads) along the microtubule tracks. Scale bar, 500 nm. B, C, Quantification of autophagosomes (B) and mitochondria (C) observed along the axon bundles in the indicated conditions; n = 3 independent microfluidic devices. *p < 0.05; n.s. not significant, Student’s t test. D, Representative electron microscopy of axon bundles of neurons cultured in microfluidic devices with (right) or without (left) BoNT/A-Hc-HRP added to the nerve terminal chambers under high K+ conditions. Autophagosomes are indicated by arrowheads. Scale bar, 500 nm. E, Quantification of BoNT/A-Hc-HRP-containing autophagosome as shown in D; n = 3. **p < 0.01, Student’s t test. F, Representative LC3 and βIII-tubulin immunostaining of hippocampal neurons cultured in microfluidic devices and fixed at indicated times after stimulation. Boxed regions are shown in G; arrowheads indicate nerve terminals. H, Quantification of the level of LC3 fluorescence normalized to βIII-tubulin; n = 6 from 2 independent cultures. ***p < 0.001; **p < 0.01, Student’s t test. I, Representative LC3 and β-actin (loading control) immunoblots. J, Quantification of I; n = 3. *p < 0.05, Student’s t test.

    Techniques Used: Electron Microscopy, Cell Culture, Immunostaining, Fluorescence, Control, Western Blot

    Related Articles

    Purification:

    Article Title: Control of Autophagosome Axonal Retrograde Flux by Presynaptic Activity Unveiled Using Botulinum Neurotoxin Type A
    Article Snippet: .. The conjugated BoNT/A-Hc-HRP was purified using a PD MiniTrap G-25 Column (GE Healthcare, catalog #28-9180-08) following the gravity protocol. .. The protein concentration was measured using the Bradford reagent (Bio-Rad, catalog #500-0006).



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    bont a hc hrp  (Cytiva Europe)
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    Cytiva Europe bont a hc hrp
    Electron microscopy of axon channels reveals that BoNT/A-Hc is retrogradely transported by autophagosomes. A, Representative axon bundles from unlabeled neurons with autophagosomes (a1 and a2, boxes) and mitochondria (arrowheads) along the microtubule tracks. Scale bar, 500 nm. B, C, Quantification of autophagosomes (B) and mitochondria (C) observed along the axon bundles in the indicated conditions; n = 3 independent microfluidic devices. *p < 0.05; n.s. not significant, Student’s t test. D, Representative electron microscopy of axon bundles of neurons cultured in microfluidic devices with (right) or <t>without</t> <t>(left)</t> <t>BoNT/A-Hc-HRP</t> added to the nerve terminal chambers under high K+ conditions. Autophagosomes are indicated by arrowheads. Scale bar, 500 nm. E, Quantification of BoNT/A-Hc-HRP-containing autophagosome as shown in D; n = 3. **p < 0.01, Student’s t test. F, Representative LC3 and βIII-tubulin immunostaining of hippocampal neurons cultured in microfluidic devices and fixed at indicated times after stimulation. Boxed regions are shown in G; arrowheads indicate nerve terminals. H, Quantification of the level of LC3 fluorescence normalized to βIII-tubulin; n = 6 from 2 independent cultures. ***p < 0.001; **p < 0.01, Student’s t test. I, Representative LC3 and β-actin (loading control) immunoblots. J, Quantification of I; n = 3. *p < 0.05, Student’s t test.
    Bont A Hc Hrp, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bont+a+hc+hrp/PD+MiniTrap+G-25/pmc04787026-49-2-11
    Average 96 stars, based on 1 article reviews
    bont a hc hrp - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Electron microscopy of axon channels reveals that BoNT/A-Hc is retrogradely transported by autophagosomes. A, Representative axon bundles from unlabeled neurons with autophagosomes (a1 and a2, boxes) and mitochondria (arrowheads) along the microtubule tracks. Scale bar, 500 nm. B, C, Quantification of autophagosomes (B) and mitochondria (C) observed along the axon bundles in the indicated conditions; n = 3 independent microfluidic devices. *p < 0.05; n.s. not significant, Student’s t test. D, Representative electron microscopy of axon bundles of neurons cultured in microfluidic devices with (right) or without (left) BoNT/A-Hc-HRP added to the nerve terminal chambers under high K+ conditions. Autophagosomes are indicated by arrowheads. Scale bar, 500 nm. E, Quantification of BoNT/A-Hc-HRP-containing autophagosome as shown in D; n = 3. **p < 0.01, Student’s t test. F, Representative LC3 and βIII-tubulin immunostaining of hippocampal neurons cultured in microfluidic devices and fixed at indicated times after stimulation. Boxed regions are shown in G; arrowheads indicate nerve terminals. H, Quantification of the level of LC3 fluorescence normalized to βIII-tubulin; n = 6 from 2 independent cultures. ***p < 0.001; **p < 0.01, Student’s t test. I, Representative LC3 and β-actin (loading control) immunoblots. J, Quantification of I; n = 3. *p < 0.05, Student’s t test.

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: Control of Autophagosome Axonal Retrograde Flux by Presynaptic Activity Unveiled Using Botulinum Neurotoxin Type A

    doi: 10.1523/JNEUROSCI.3757-14.2015

    Figure Lengend Snippet: Electron microscopy of axon channels reveals that BoNT/A-Hc is retrogradely transported by autophagosomes. A, Representative axon bundles from unlabeled neurons with autophagosomes (a1 and a2, boxes) and mitochondria (arrowheads) along the microtubule tracks. Scale bar, 500 nm. B, C, Quantification of autophagosomes (B) and mitochondria (C) observed along the axon bundles in the indicated conditions; n = 3 independent microfluidic devices. *p < 0.05; n.s. not significant, Student’s t test. D, Representative electron microscopy of axon bundles of neurons cultured in microfluidic devices with (right) or without (left) BoNT/A-Hc-HRP added to the nerve terminal chambers under high K+ conditions. Autophagosomes are indicated by arrowheads. Scale bar, 500 nm. E, Quantification of BoNT/A-Hc-HRP-containing autophagosome as shown in D; n = 3. **p < 0.01, Student’s t test. F, Representative LC3 and βIII-tubulin immunostaining of hippocampal neurons cultured in microfluidic devices and fixed at indicated times after stimulation. Boxed regions are shown in G; arrowheads indicate nerve terminals. H, Quantification of the level of LC3 fluorescence normalized to βIII-tubulin; n = 6 from 2 independent cultures. ***p < 0.001; **p < 0.01, Student’s t test. I, Representative LC3 and β-actin (loading control) immunoblots. J, Quantification of I; n = 3. *p < 0.05, Student’s t test.

    Article Snippet: The conjugated BoNT/A-Hc-HRP was purified using a PD MiniTrap G-25 Column (GE Healthcare, catalog #28-9180-08) following the gravity protocol.

    Techniques: Electron Microscopy, Cell Culture, Immunostaining, Fluorescence, Control, Western Blot